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AAT Bioquest

FAQ

Posted on April 12, 2024
What are the consequences of under-fixation or over-fixation?
BioconjugationAntibodies and ProteomicsAntibody and Protein LabelingCellular Structures and OrganellesChromatin Immunoprecipitation (ChIP)DNA and RNA QuantitationNucleic AcidsNucleus


Posted on August 29, 2023
What are antibodies made of?
Antibodies and ProteomicsAntibody and Protein Labeling


Posted on June 21, 2023
What are the advantages of using mass spectrometry (MS) to detect phosphorylated Proteins?
Antibodies and ProteomicsAntibody and Protein LabelingChemical ReagentsPhysiological ProbesProtein


Posted on November 23, 2022
What is CD3 a marker of?
BioconjugationAntibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on November 23, 2022
What immune cells express CD14?
Antibodies and ProteomicsAntibody and Protein LabelingBiocojugationCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on November 23, 2022
What are the functions of CD14?
BioconjugationAntibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on November 23, 2022
What cells express CD34?
BioconjugationAntibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on November 23, 2022
What is CD34?
BioconjugationAntibodies and ProteomicsAntibody and Protein LabelingCluster of Differentiation (CD Antibodies)Immunohistochemistry (IHC)


Posted on November 9, 2022
What are the similarities between B cell receptor and antibody?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugationCellular Processes


Posted on October 14, 2022
Is propidium iodide cell permeable?
Antibodies and ProteomicsBioconjugationCell Cycle AssaysCell Structures and Organelles


Posted on October 7, 2022
What are the differences between polyclonal and monoclonal antibodies?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugation


Posted on October 7, 2022
What are recombinant antibodies?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugationImmunohistochemistry (IHC)


Posted on August 29, 2022
What is an Immunoglobulin?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)


Posted on August 29, 2022
What are the similarities between immunoglobulin and antibody?
BioconjugationAntibodies and ProteomicsAntibody and Protein Labeling


Posted on August 25, 2022
What are the differences between phage display and hybridoma?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugationImmunohistochemistry (IHC)


Posted on August 17, 2022
What are the main types of antibodies?
Antibody and Protein LabelingAntibodies and ProteomicsImmunohistochemistry (IHC)Antibody and Protein LabelingBioconjugation


Posted on August 17, 2022
What should be considered when generating monoclonal antibodies?
BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)Antibodies and ProteomicsPhysiological Probes


Posted on August 5, 2022
What are the differences between flow cytometry and immunohistochemistry?
Flow Cytometry ReagentsImmunohistochemistry (IHC)BioconjugationAntibodies and ProteomicsProtein to Protein Conjugation


Posted on August 2, 2022
What are the differences between B cell receptor and antibody?
Antibody and Protein LabelingBioconjugationAntibodies and ProteomicsImmunohistochemistry (IHC)


Posted on July 27, 2022
What are the disadvantages of polyclonal antibodies?
Cellular ProcessesAntibody and Protein LabelingBioconjugationAntibodies and ProteomicsCrosslinkers


Posted on July 25, 2022
What are the functions of immunoglobulin?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Physiological Probes


Posted on July 25, 2022
What are the primary classes of immunoglobulins?
Antibodies and ProteomicsImmunohistochemistry (IHC)Antibody and Protein LabelingPhysiological Probes


Posted on July 21, 2022
What are the applications of phage display technology?
BioconjugationChemical ReagentsAntibodies and ProteomicsAntibody and Protein Labeling


Posted on July 21, 2022
What are the types of combinatorial antibody libraries?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugation


Posted on July 21, 2022
What is a hybridoma cell used for?
BioconjugationAntibody and Protein LabelingAntibodies and Proteomics


Posted on July 21, 2022
What does phage display detect?
Antibody and Protein LabelingProtein to Protein ConjugationAntibodies and ProteomicsBioconjugation


Posted on July 21, 2022
How are recombinant antibodies produced?
Antibody and Protein LabelingAntibodies and ProteomicsBioconjugation


Posted on July 8, 2022
Is CD45 a phosphatase?
Physiological ProbesAntibody LabelingAntibodies and ProteomicsCell Cytotoxicity


Posted on July 8, 2022
What is the significance of CD markers on cells?
Flow Cytometry ReagentsAntibody LabelingAntibodies and ProteomicsPhysiological Probes


Posted on July 8, 2022
Is CD45 a cell surface marker?
Flow Cytometry ReagentsAntibody LabelingAntibodies and ProteomicsPhysiological Probes


Posted on July 8, 2022
What cell has CD45?
Physiological ProbesAntibody LabelingAntibodies and ProteomicsFlow Cytometry Reagents


Posted on July 8, 2022
Where is CD45 found?
Flow Cytometry ReagentsAntibodies and ProteomicsAntibody LabelingPhysiological Probes


Posted on June 30, 2022
What are monoclonal antibodies?
Antibodies and ProteomicsImmunohistochemistry (IHC)Enzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein Labeling


Posted on June 30, 2022
What are polyclonal antibodies?
BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)Enzyme-Linked Immunosorbent Assay (ELISA)Antibodies and Proteomics


Posted on June 30, 2022
What are the advantages of using primary antibodies?
Antibodies and ProteomicsEnzyme-Linked Immunosorbent Assay (ELISA)Immunohistochemistry (IHC)Antibody and Protein LabelingBioconjugation


Posted on June 30, 2022
What are the advantages of using secondary antibodies?
Antibody and Protein LabelingBioconjugationEnzyme-Linked Immunosorbent Assay (ELISA)Immunohistochemistry (IHC)Antibodies and Proteomics


Posted on June 30, 2022
What is the difference between a primary antibody and a secondary antibody?
Antibodies and ProteomicsProtein to Protein ConjugationAntibody and Protein LabelingPhysiological Probes


Posted on June 29, 2022
What are the advantages of recombinant antibodies?
Physiological ProbesAntibody and Protein LabelingImmunohistochemistry (IHC)Antibodies and Proteomics


Posted on June 29, 2022
How are recombinant antibodies made?
Antibodies and ProteomicsDNA and RNA QuantitationAntibody and Protein LabelingBioconjugationPhysiological Probes


Posted on June 29, 2022
What are the common types of antibodies?
Immunohistochemistry (IHC)Antibodies and ProteomicsAntibody and Protein LabelingBioconjugation


Posted on June 28, 2022
What are the types of recombinant antibody fragments?
Antibody and Protein LabelingEnzyme-Linked Immunosorbent Assay (ELISA)Antibodies and ProteomicsImmunohistochemistry (IHC)Bioconjugation


Posted on June 28, 2022
How do I select the right secondary antibodies?
Antibodies and ProteomicsEnzyme-Linked Immunosorbent Assay (ELISA)Immunohistochemistry (IHC)Bioconjugation


Posted on June 27, 2022
What are the main differences between a primary antibody and a secondary antibody?
Antibody and Protein LabelingBioconjugationAntibodies and ProteomicsEnzyme-Linked Immunosorbent Assay (ELISA)


Posted on June 24, 2022
What are the common types of primary antibodies?
Antibodies and ProteomicsEnzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein LabelingBioconjugation


Posted on June 24, 2022
What are the most common types of secondary antibodies?
BioconjugationAntibodies and ProteomicsEnzymesEnzyme-Linked Immunosorbent Assay (ELISA)Antibody and Protein Labeling


Posted on June 24, 2022
How do primary and secondary antibodies work in immunoassays?
Antibodies and ProteomicsEnzyme-Linked Immunosorbent Assay (ELISA)Immunohistochemistry (IHC)Antibody and Protein LabelingPhysiological Probes


Posted on June 24, 2022
How do I choose primary and secondary antibodies?
BioconjugationAntibody and Protein LabelingAntibodies and Proteomics


Posted on May 24, 2022
What is the difference between mass spectroscopy and mass spectrometry?
Classic DyesBioconjugationAntibodies and ProteomicsAntibody and Protein Labeling


Posted on March 25, 2022
What are the advantages of fragment analysis?
Antibodies and ProteomicsAntibody and Protein LabelingChemical ReagentsDNA and RNA Quantitation


Posted on March 18, 2022
What are the common affinity tags for protein purification?
BioconjugationPhysiological ProbesAntibody and Protein LabelingAntibodies and Proteomics


Posted on March 18, 2022
Why is NaCl used in size exclusion chromatography?
Physiological ProbesAntibodies and ProteomicsAntibody and Protein Labeling


Posted on March 18, 2022
How buffer pH and NaCl affect size exclusion chromatography?
Physiological ProbesBioocnjugationAntibodies and ProteomicsAntibody and Protein Labeling


Posted on March 18, 2022
What is EDTA used for in chemistry?
Physiological ProbesBioconjugationAntibodies and Proteomics


Posted on March 18, 2022
What is Column Chromatography?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugation


Posted on March 18, 2022
What is Chromatography?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugation


Posted on March 18, 2022
What are the main phases of Column Chromatography?
Antibodies and ProteomicsAntibody and Protein LabelingPhysiological ProbesBioconjugation


Posted on March 18, 2022
What are the commonly used chromatographic methods for protein purification?
BioconjugationPhysiological ProbesAntibody and Protein LabelingAntibodies and Proteomics


Posted on March 16, 2022
Why is Protein Purification Important?
Antibodies and ProteomicsAntibody and Protein LabelingChemical Reagents


Posted on March 16, 2022
What are the common methods for protein purification?
Chemical ReagentsClassic DyesAntibodies and ProteomicsAntibody and Protein Labeling


Posted on March 16, 2022
How Does Protein Purification Work?
Antibodies and ProteomicsAntibody and Protein LabelingChemical ReagentsCrosslinkers


Posted on March 11, 2022
Why is EDTA used in protein purification?
BioconjugationPhysiological ProbesAntibodies and Proteomics


Posted on March 11, 2022
Which chromatography is best for protein purification?
BioconjugationAntibodies and ProteomicsAntibody and Protein Labeling


Posted on March 11, 2022
How does column chromatography work?
Antibodies and ProteomicsAntibody and Protein LabelingPhysiological ProbesBioconjugation


Posted on March 11, 2022
How does affinity purification work?
Antibodies and ProteomicsAntibody and Protein LabelingPhysiological ProbesBioconjugation


Posted on January 6, 2022
What do the peaks in mass spectrometry represent?
Antibodies and ProteomicsPhysiological ProbesBioconjugation


Posted on December 23, 2021
What are the differences between ion exchange chromatography and affinity chromatography?
Antibodies and ProteomicsBioconjugationAntibody and Protein LabelingProtein to Protein Conjugation


Posted on December 16, 2021
What is the difference between an isocratic and a gradient elution method?
Antibodies and ProteomicsBioconjugationPhysiological Probes


Posted on December 15, 2021
What elutes first in affinity chromatography?
BioconjugationPhysiological ProbesAntibodies and Proteomics


Posted on December 14, 2021
What is Immobilized Metal Chelate Affinity Chromatography (IMAC)?
Physiological ProbesAntibodies and ProteomicsAntibody and Protein Labeling


Posted on December 14, 2021
What are the applications of immobilized metal affinity chromatography (IMAC)?
BioconjugationAntibody and Protein LabelingAntibodies and Proteomics


Posted on October 27, 2021
How do I choose the right fluorophore?
Antibodies and ProteomicsiFluor Dyes and KitsClassic Dyes


Posted on October 12, 2021
Is AIM2 an NLR protein?
Antibodies and ProteomicsCell Viability


Posted on October 11, 2021
What is the AIM2 inflammasome?
Antibodies and ProteomicsApoptosis and NecrosisCaspases


Posted on September 29, 2021
What is secondary antibody cross reactivity?
Secondary AntibodiesAntibodies and Proteomics


Posted on September 28, 2021
What is the concentration of secondary antibodies for immunofluorescence?
Secondary AntibodiesAntibodies and ProteomicsImmunohistochemistry (IHC)Power Styramide™ Signal Amplification (PSA™)


Posted on September 28, 2021
What does cross adsorbed secondary antibody mean?
Secondary AntibodiesAntibodies and ProteomicsPrimary AntibodiesIndocyanine Green


Posted on September 28, 2021
How long do secondary antibodies last?
Secondary AntibodiesAntibodies and Proteomics


Posted on September 28, 2021
How long do primary antibodies last?
Primary AntibodiesAntibodies and Proteomics


Posted on September 28, 2021
Are primary antibodies protein specific?
Antibodies and ProteomicsPrimary Antibodies


Posted on September 27, 2021
How do I know if an antibody is primary or secondary?
EnzymesSecondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRP


Posted on September 27, 2021
What does a conjugated antibody mean?
Horseradish Peroxidase (HRP) and Poly-HRPPE and APCSecondary AntibodiesAntibodies and Proteomics


Posted on September 27, 2021
What are the types of IgG?
Secondary AntibodiesAntibodies and Proteomics


Posted on September 27, 2021
What is the advantage of using the pre-adsorbed secondary antibody?
Secondary AntibodiesAntibodies and ProteomicsImmunohistochemistry (IHC)


Posted on September 23, 2021
What is the difference between cross adsorbed/normal/purified antibodies?
Antibodies and ProteomicsSecondary Antibodies


Posted on September 23, 2021
What are the major benefits of using a secondary antibody
Antibodies and ProteomicsPrimary AntibodiesSecondary Antibodies


Posted on September 22, 2021
What label should I choose for a secondary antibody?
Secondary AntibodiesAntibodies and ProteomicsImmunohistochemistry (IHC)


Posted on September 22, 2021
What is the most important criterion for choosing a secondary antibody?
Secondary AntibodiesAntibodies and Proteomics


Posted on September 22, 2021
How do I choose secondary antibodies?
Secondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRPEnzyme-Linked Immunosorbent Assay (ELISA)


Posted on September 22, 2021
Why is an HRP conjugated secondary antibody used in western blotting?
Horseradish Peroxidase (HRP) and Poly-HRPEnzymesSecondary AntibodiesAntibodies and Proteomics


Posted on September 21, 2021
What does HRP bind to?
BioconjugationAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRPProtein to Protein Conjugation


Posted on September 21, 2021
Is IgG a secondary antibody?
Secondary AntibodiesAntibodies and ProteomicsPower Styramide™ Signal Amplification (PSA™)


Posted on September 21, 2021
What do anti-human antibodies bind to?
Secondary AntibodiesAntibodies and Proteomics


Posted on September 21, 2021
Are there different IgG antibodies?
Antibodies and ProteomicsImmunohistochemistry (IHC)Secondary Antibodies


Posted on September 21, 2021
What do anti-human antibodies do?
Secondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRP


Posted on September 21, 2021
What is anti human IgG?
Secondary AntibodiesAntibodies and ProteomicsHorseradish Peroxidase (HRP) and Poly-HRP


Posted on September 20, 2021
What are IgG antibodies used for?
Antibodies and ProteomicsAntibody and Protein LabelingSecondary Antibodies


Posted on May 26, 2021
What is the difference between immunoprecipitation and coimmunoprecipitation?
BioconjugationAntibodies and ProteomicsImmunohistochemistry (IHC)


Posted on May 18, 2021
What is reciprocal immunoprecipitation?
Antibodies and ProteomicsAntibody and Protein LabelingBioconjugation


Posted on December 4, 2020
What are the applications of tissue culture?
Antibodies and ProteomicsCell Cycle and Proliferation


Posted on December 1, 2020
What are the reasons for high background in ELISA?
ELISAAntibodies and ProteomicsEnzyme-Linked Immunosorbent Assay (ELISA)Immunohistochemistry (IHC)BioconjugationHorseradish Peroxidase (HRP) and Poly-HRP


Posted on April 17, 2020
What is IHC double staining?
Antibodies and ProteomicsAntibody and Protein LabelingImmunohistochemistry (IHC)Chemical ReagentsClassic DyesIHCPhysiological Probes


Posted on April 2, 2020
What are the roles of CD4 and CD8 in T cell activation?
Antibodies and ProteomicsAntibody and Protein LabelingCD4+ T CellsCluster of Differentiation (CD Antibodies)IHCImmunohistochemistry (IHC)